cDNA Laboratory, cDNA; cDNA library; cDNA library construction

cDNA Lab > Standard cDNA Library > Standard Plasmid cDNA Libraries > pcDNALab Vector

Standard Plasmid, pcDNALAB Vector Construction

           The oligo (dT)₂₅primer was used for the 1st strand cDNA synthesis. followed by 2^nd strand cDNA synthesis and additional processing, finally the cDNA fragments were cloned into the vector pcDNALAB.

The pcDNALAB vectors can be used for sense strand RNA generates by in vivo transcription and production of stably transfected cell lines.

The cDNA Library Propertise                                         Picture

Standard

Starting Material

Total RNA (≥0.5mg)
Cells (≥1.0 x1 0⁸)

Tissue (≥0.5 g)

Primary Clones

1 x 10^6~8 cfu/ml

Insert Size

0.5 ~6.0 kb

% Recombinants

>95%

The pDNR is a donor vector in the Creator^TM Cloning System. The clones can be transferred into many acceptor vectors, which suitable for mammalian and prokaryotic expression; two-hybrid screening system, etc. See (www.clonetech.com).

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