The pcDNA3.1 vectors can be used for mammalian expression, sense strand RNA generates by in vivo transcription and production of stably transfected cell lines. See the vector map and multiple cloning site of pcDNA3.1 (+) at:(www.invitrogen.com/content/sfs/manuals/pcdna3.1_man.pdf)
The cDNA Library Propertise Picture
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Standard
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Starting Material
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Total RNA (≥0.5mg)
Cells (≥1.0 x1 08)
Tissue (≥0.5 g)
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Primary Clones
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1 x 106~8 cfu/ml
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Insert Size
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0.5 ~6.0 kb
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% Recombinants
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>95%
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The pDNR is a donor vector in the CreatorTM Cloning System. The clones can be transferred into many acceptor vectors, which suitable for mammalian and prokaryotic expression; two-hybrid screening system, etc. See (www.clonetech.com)
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